co-integrate vector system

A two plasmid system for plant transgenesis. One plasmid is engineered to carry a T-DNA segment incorporating the gene(s) to be introduced. After introduction into Agrobacterium tumefaciens, the plasmid undergoes homologous recombination with a resident disarmed Ti plasmid to form a single plasmid carrying the genetic information for transferring the genetically engineered T-DNA region to plant cells.