co-transformation

A protocol for producing transgenesis, in which host (plant or animal) cells are transformed simultaneously with two different plasmids, one of which carries a selectable marker, and the other the gene to be transferred. Relies on the observation that given a sufficiently high concentration of both plasmids, transformed cells will have incorporated both plasmids, possibly at different genomic loci. If the transgenes are separable through normal meiotic recombination, transgenic individuals without the selectable marker can be selected in subsequent generations.