narrow-host-range plasmidA plasmid that can replicate in one, or at most a few, different bacterial species.
narrow-sense heritabilityThe proportion of the phenotypic variance that is due to variation in breeding values; the proportion of the phenotypic variance that is due to additive…
National Science Foundation (NSF)A nonregulatory agency which has oversight of biotechnology research activities that the agency funds.
native proteinThe naturally occurring form of a protein.
Natural selectionThe differential survival and reproduc- tion of organisms with genetic characteristics that enable them to better utilize environmental resources.
necrosis(ne-KRO-sis) Refers to the death of living tissues.
negative autogenous regulationInhibition of the expression of a gene or set of co-ordinately regulated genes by the product of the gene or the product of one of the genes.
negative control systemA mechanism by which a regulatory protein is required to turn off gene expression.
negative selectionSelection against individuals possessing a certain character. Opposite: positive selection.
neomycin phosphotransferase II(Abbreviation: npt-II). An enzyme which detoxifies the antibiotic neomycin, used as a marker gene to select for successfully transformed cells in plant…
neoplasm(NEE-oh-PLA-zum) An abnormal mass of tissue that results when cells divide more than they should or do not die when they should.
nickTo break (or a break in) a phosphodiester bond in one of the strands of a double-stranded DNA molecule.
Nick translationA procedure for making a DNA probe in which a DNA fragment is treated with DNase to produce single-stranded nicks, followed by incorporation of…
nicked circleDuring the extraction of plasmid DNA from a bacterial cell, one strand of the DNA often becomes nicked.
Nicked circle (relaxed circle)During extraction of plasmid DNA from the bacterial cell, one strand of the DNA becomes nicked.
nif gene clusterGroup of bacterial genes responsible for the biological fixation of atmospheric nitrogen.
NIHA federal agency in the U.S. that conducts biomedical research in its own laboratories; supports the research of non-Federal scientists in universities…
nitrateThe form of nitrogen that can be used directly by plants; a major component of inorganic fertilizers.
nitrificationThe natural process in which nitrogen in plant and animal wastes is oxidized, first to nitrites and then to nitrates, through the action of soil-borne…
NitrocelluloseA membrane used to immobilize DNA, RNA, or protein, which can then be probed with a labeled sequence or antibody.
nitrogen assimilationThe incorporation of nitrogen into the cells of living organisms.
Nitrogen fixationThe conversion of atmospheric nitrogen to biologically usable nitrates.
Nitrogenous baseThe purines (adenine and guanine) and pyrimidines (cytosine, and thymine or uracil ) that are present in DNA and RNA.
Nitrogenous basesThe purines (adenine and guanine) and pyrimidines (thymine, cytosine, and uracil) that comprise DNA and RNA molecules.
nod boxA DNA sequence that controls the transcriptional regulation of Rhizobium nodulation genes.
nodal cultureThe culture of a lateral bud and a section of adjacent stem tissue.
nodeAny device connected to network. PCs, servers, and printers are all nodes on the network.
nodularTerm commonly used to describe the pebbly (rough) texture of a callus.
nodulationThe formation of nodules on the roots of legumes following colonization by symbiotic nitrogen-fixing bacteria.
nodule(NOD-yool) A growth or lump that may be cancerous or noncancerous.
non repetitive DNA/RNAA nucleotide sequence which does not include a significant proportion of repetitive sequences of nucleotide s.
non-additive genetic variationThe proportion of the total genetic variation in a population that does not respond to simple mass selection and that causes specific pairwise crosses to…
non-autonomousA term referring to biological units that cannot function by themselves; such units require the assistance of another unit, or "helper".
non-disjunctionFailure of separation of homologous chromosomes or chromatids in mitosis or meiosis, resulting in too many chromosomes in some daughter cells and too few…
Northern hybridization(Northern blotting). A procedure in which RNA fragments are transferred from an agarose gel to a nitrocellulose filter, where the RNA is then hybridized…
npt-IIAbbreviation for neomycin phosphotransferase II.
NSFLotus Notes Format Database File (i.e. database.nsf) Can be either an email database or the traditional type of fielded database.
nucellar embryoAn embryo which has developed vegetatively from somatic tissue surrounding the embryo sac, rather than by fertilization of the egg cell.
nucellusTissue forming the major part of the young ovule in which the embryo sac develops; Also known as megasporangium.
Nuclear reprogrammingRestoration of the correct embryonic pattern of gene expression in a nucleus derived from a somatic cell and introduced into an oocyte.
Nuclear transferA technology by which novel animals are generated by cloning a single diploid somatic cell.
Nuclear transfer (NT)The generation of a new animal nearly identical to another one by injection of the nucleus from a cell of the donor animal into an enucleated oocyte of…
NucleaseA class of enzymes that degrades DNA and/or RNA molecules by cleaving the phosphodiester bonds that link adjacent nucleotides.
Nucleic acidA macromolecule consisting of polymerized nucleotides . Two forms are found, DNA and RNA.
Nucleic acidsThe two nucleic acids, deoxyribonucleic acid (DNA) and ribonucleic acid (RNA), are made up of long chains of molecules called nucleotides.
NucleinThe term used by Friedrich Miescher to describe the nuclear material he discovered in 1869, which today is known as DNA.
nucleo-cytoplasmic ratioIn a cell, the ratio of nuclear to cytoplasmic volume. This ratio is high in meristematic cells and low in differentiated cells.
nucleolar organizer region(Abbreviation: NOR). A chromosomal segment containing a large array of genes that encode ribosomal RNA; located at the secondary constriction of specific…
nucleolusAn RNA -rich nuclear organelle in the nucleus of eukaryotic cells, produced by a nucleolar organizer.
nucleoplasmThe non-staining or slightly chromophilic, liquid or semi-liquid, ground substance of the interphase nucleus and which fills the nuclear space around the…
nucleoproteinConjugated protein composed of nucleic acid and protein; the material of which the chromosomes are made.
NucleosideA building block of DNA and RNA, consisting of a nitrogenous base linked to a five carbon sugar. (See Nucleoside analog.)
Nucleoside analogA synthetic molecule that resembles a naturally occuring nucleoside, but that lacks a bond site needed to link it to an adjacent nucleotide.
nucleoside analogueA synthetic molecule that resembles a naturally occurring nucleoside.
nucleosomeSpherical sub-units of eukaryotic chromatin that are composed of a core particle consisting of an octamer of histones (two molecules each of histones H2a…
Nucleotide(NOO-klee-oh-TIDE) A building block for nucleic acids (the molecules inside cells that carry genetic information).
nullisomyAn otherwise diploid cell or organism lacking both members of a homologous chromosome pair.
nurse cultureCulturing cells from a suspension culture on a raft of filter paper above a callus tissue piece (nurse tissue).
nutriceuticalA conventional food product that has been modified (potentially by genetic engineering) to have improved nutritional characteristics and/or pharmaceutical…
nutrient cycleThe passage of a nutrient or element through an ecosystem, including its assimilation and release by various organisms and its transformation into various…
nutrient deficiencyAbsence or insufficiency of an essential factor for normal growth and development.
nutrient film technique(Abbreviation: NFT). A hydroponic technique used to grow plants. NFT delivers a thin film of water or nutrient solution either continuously or through…
nutrient gradientA diffusion gradient of nutrients and gases that develops in tissues where only a portion of the tissue is in contact with the medium.
nutrient medium(pl.: nutrient media) A solid, semi-solid or liquid formulation for in vitro cell or tissue growth.