R genesA class of plant genes conferring resistance to a specific strain (or group of strains) of a particular pathogen.
R-loopsSingle-stranded DNA regions in RNA -DNA hybrids formed in vitro under conditions where RNA -DNA duplexes are more stable than DNA -DNA duplexes.
R1The first-generation offspring of a recombinant (genetically modified) organism. Not standard terminology. See: T0, T1, and T2.
RACEA distinguishable group of organisms of a particular species. Criteria for distinctness can be one or a combination of geographic, ecological…
racemeAn inflorescence in which the main axis is elongated but the flowers are borne on pedicels that are about equal in length.
rachisMain axis of a spike; axis of fern leaf (frond) from which pinnae arise; in compound leaves, the extension of the petiole corresponding to the midrib of…
radiation hybrid cell panel(Abbreviation: RH). A somatic cell hybrid panel in which the chromosomes from the species of interest have been fragmented by irradiation prior to cell…
radicleThe portion of the plant embryo which develops into the primary root.
Radioimmunoassay(Abbreviation: RIA). An assay based on the use of a radioactively labelled antibody, where the amount of radiation detected indicates the amount of target…
radioisotope(RAY-dee-oh-I-suh-tope) An unstable form of a chemical element that releases radiation as it breaks down and becomes more stable.
rametAn individual member of a clone, descended from the ortet.
random amplified polymorphic DNA(Abbreviation: RAPD). A PCR -based genotyping technique in which genomic template is amplified with single, short (usually 10-mer) randomly chosen primers.
random mutagenesisA non-directed change of one or more nucleotide pairs in a DNA molecule.
random primer methodA method for labelling DNA probes, mainly for Southern hybridization experiments.
RAPDAbbreviation for random amplified polymorphic DNA.
rate-limiting enzymeThe enzyme whose activity controls the output of final product from a multi-enzyme metabolic pathway.
rational drug designA systematic method of creating compounds by analysing their structure, function and stereochemical interactions.
read-throughTranscription or translation that proceeds beyond the normal stopping point because of the absence of the usual transcription or translation termination…
Reading frameA series of triplet codons beginning from a specific nucleotide. Depending on where one begins, each DNA strand contains three different reading frames.
recAA protein, found in most bacteria, that is essential for DNA repair and DNA recombination.
recalcitrantOf seeds, unable to survive drying and subsequent storage at low temperature. See: field gene bank.
receptacleEnlarged end of the pedicel or peduncle, to which other flower parts are attached.
receptor(reh-SEP-ter) A molecule inside or on the surface of a cell that binds to a specific substance and causes a specific physiologic effect in the cell.
receptor-binding screeningA biotechnology-based method for drug discovery, which relies on the fact that many drugs act by binding to specific receptors on or in cells.
recessiveDescribing an allele whose effect with respect to a particular trait is not evident in heterozygotes. Opposite: dominant.
recessive alleleAllelic state of a gene, where homozygosity is required for the expression of the relevant phenotype. Opposite:.
Recessive geneCharacterized as having a phenotype expressed only when both copies of the gene are mutated or missing.
recessive oncogeneA single copy of this gene is sufficient to suppress cell proliferation; the loss of both copies of the gene contributes to cancer formation.
Recessive(-acting) oncogene, (anti-oncogene)A single copy of this gene is sufficient to suppress cell proliferation; the loss of both copies of the gene contributes to cancer formation.
Recognition sequence (site)A nucleotide sequence--composed typically of 4, 6, or 8 nucleotides--that is recognized by a restriction endonuclease.
recognition siteA nucleotide sequence, typically 4-8bp long and often palindromic, that is recognized by, and at which a restriction endonuclease binds to the DNA.
recombinant(ree-KOM-bih-nunt) In genetics, describes DNA, proteins, cells, or organisms that are made by combining genetic material from two different sources.
recombinant DNAThe process of cutting and recombining DNA fragments from different sources as a means to isolate genes or to alter their structure and function.
Recombinant DNA molecules (rDNA)A combination of DNA molecules of different origin that are joined using recombinant DNA technologies.
Recombinant DNA technologyA set of techniques for manipulating DNA, including: the identification and cloning of genes; the study of the expression of cloned genes; and the…
recombinant human(Abbreviation rh). A prefix denoting molecules made through the use of recombinant DNA technology.
recombinant proteinA protein encoded by a cloned gene. Synonym: heterologous protein.
recombinant RNARNA molecules joined in vitro by T4 RNA ligase.
recombinant toxinA single multifunctional toxic protein encoded by a recombinant gene.
recombinaseA class of enzymes that are able to alter the arrangement of DNA sequences in a site-specific way.
RecombinationThe production of a DNA molecule with segments derived from more than one parent DNA molecule.
recombination fractionThe proportion of recombinant (with respect to two loci) gametes arising from meiosis.
Recombination frequencyThe frequency at which crossing over occurs between two chromosomal loci--the probability that two loci will become unlinked during meiosis.
recombinational hot spotA chromosomal region where recombination appears to occur more frequently than expected.
reconstructed cellA viable transformed cell resulting from genetic engineering.
reduction divisionThe first division of meiosis in which the chromosome number is reduced from the somatic to the gametic number.
refugium(pl.: refugia) An area set aside to provide protection/escape from ecological consequences occurring elsewhere.
regenerationIn biology, regrowth of damaged or destroyed tissue or body part.
regulatorSubstance regulating growth and development of cells, organs, etc.
Regulatory geneA gene whose protein controls the activity of other genes or metabolic pathways.
regulatory sequenceA DNA sequence involved in regulating the expression of a gene, e.g. a promoter or operator region (in the DNA molecule).
rejuvenation1. Reversion from adult to juvenile stage. 2. The process of regular reproduction of seed stocks or collections in gene banks, in order to ensure…
Relaxed plasmidA plasmid that replicates independently of the main bacterial chromosome and is present in 10-500 copies per cell. (See Plasmid.)
release factor1. A soluble protein that recognizes termination codon s in mRNA s and terminates translation in response to these codons. 2.
RemediationThe cleanup or containment of a hazardous waste disposal site to the satisfaction of the applicable regulatory agency.
renaturationOf DNA, the reforming of two complementary molecules into a double-stranded structure, following heat or chemical induction of dissociation (denaturation).
RenatureThe reannealing (hydrogen bonding) of single- stranded DNA and/or RNA to form a duplex molecule.
renninAn enzyme, secreted by cells lining the stomach in mammals, responsible for the clotting of milk. Used in the manufacture of certain dairy products.
repeat unitA sequence of nucleotide s that occurs repeatedly, often in a head-to-tail arrangement (tandemly).
Repetitive DNADNA sequences that are present in a genome in many copies, some of it originating from retrotransposon activity.
replacementThe addition of a cloned corrected copy of a defective gene. See: homogenotization.
replacement therapyThe administration of metabolites, co-factors or hormones that are deficient as the result of a genetic disease.
replica platingDuplicating a population of bacterial colonies growing on agar medium in one Petri plate to agar medium in another Petri plate.
replicaseA viral enzyme necessary for the replication of the virus in the host cell.
ReplicationThe in vivo synthesis of double-stranded DNA by copying from a single-stranded template.
replication forkY shaped structure associated with DNA replication. It represents the point at which the strands of double-stranded DNA are separated so that replication…
replicative form(Abbreviation: RF). The molecular configuration of viral nucleic acid that is the template for replication in the host cell.
RepliconA chromosomal region containing the DNA sequences necessary to initiate DNA replication processes.
replisomeThe complete replication apparatus, present at a replication fork, that carries out the replication of DNA.
Reporter geneA gene that encodes a product that can be readily assayed. Used as to confirm the incorporation of a transgene into, organ or tissue, and as a means of…
repressible enzymeAn enzyme whose activity can be diminished by the presence of a regulatory molecule.
repressible geneA gene whose expression can be diminished or extinguished by the presence of a regulatory molecule.
repressionInhibition of transcription by preventing RNA polymerase from binding to the transcription initiation site.
repressorA DNA-binding protein in prokaryotes that blocks gene transcription by binding to the operator.
reproduction1. Sexual reproduction: the regular alteRNA tion of meiosis and fertilization which provides for the production of offspring.
repulsionA double heterozygote in which the dominant (or wild-type) allele at one locus and the recessive (or mutant) allele at a second linked locus occur on the…
residue1. See: polymer. 2. Materials remaining after degradation and/or attempted removal, e.g. pesticide residues in food.
ResistanceThe ability of an organism to develop ways to be impervious to specific threats to their existence.
resistance factorA plasmid that confers antibiotic resistance to a bacterium.
Resistance managementStrategies that can delay the onset of resistance. For insect resistance management, this includes the use of a refuge in which the insect will not be…
rest periodA physiological condition of viable seed s, buds or bulbs that prevents growth even in the presence of otherwise favourable environmental conditions.
restitution nucleusA single nucleus arising from a failure of nuclear division, either during meiosis, in which a gamete is formed with the unreduced chromosome number; or…
restriction endonucleaseA class of enzymes that cut DNA after recognizing a specific sequence. The three types of restriction endonuclease are: I.
Restriction endonuclease (enzyme)A class of endonucleases that cleaves DNA after recognizing a specific sequence, such as BamH1 (GGATCC), EcoRI (GAATTC), and HindIII (AAGCTT). Type I.
restriction exonucleaseA class of enzymes that degrade DNA or RNA, starting from either the 5'- or the 3'-end.
restriction fragmentA shortened DNA molecule generated by the cleavage of a larger molecule by one or more restriction endonucleases.
restriction fragment length polymorphism(Abbreviation: RFLP). A class of genetic marker based on the detection of variation in the length of restriction fragments generated when DNA is treated…
Restriction fragment length polymorphism (RFLP)Differences in nucleotide sequence between alleles at a chromosomal locus result in restriction fragments of varying lengths detected by Southern analysis.