Nick translation

1 topic · 2 sources

Science› Biotechnology and Genetics

Food Biotechnology

A procedure for making a DNA probe in which a DNA fragment is treated with DNase to produce single-stranded nicks, followed by incorporation of radioactive nucleotides from the nicked sites by DNA polymerase I.

A procedure for labelling DNA by treating a fragment with DNAse to produce single-stranded nicks followed by excision of a nucleotide and repair of the gaps with radiolabelled nucleotide.

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