Nick translation
Science› Biotechnology and Genetics
Food Biotechnology
A procedure for making a DNA probe in which a DNA fragment is treated with DNase to produce single-stranded nicks, followed by incorporation of radioactive nucleotides from the nicked sites by DNA polymerase I.
A procedure for labelling DNA by treating a fragment with DNAse to produce single-stranded nicks followed by excision of a nucleotide and repair of the gaps with radiolabelled nucleotide.