Km 3 topics · 3 sources Science› Biotechnology and GeneticsFood BiotechnologyA dissociation constant that characterizes the binding of an enzyme to a substrate. The smaller the value of Km, the tighter the binding of the enzyme to the substrate. Also called the Michaelis constant.UN Food and Agriculture Organization — Glossary of Biotechnology for Food and Agriculture Cite TechnologyDefenseKilometer.DoD Missile Defense Agency — MDA Glossary Cite TechnologyTelecommunicationsAbbreviation for kilometer.ATIS — ATIS Telecom Glossary 2007 Cite