phosphodiester (phospho-diester) bond 1 topic · 1 source Science› Biotechnology and GeneticsFood BiotechnologyA bond in which a phosphate group joins adjacent carbons through ester linkages. A condensation reaction between adjacent nucleotides results in a phosphodiester bond between 3' and 5' carbons in DNA and RNA.UN Food and Agriculture Organization — Glossary of Biotechnology for Food and Agriculture Cite