melting temperature

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Food Biotechnology

(Abbreviation: T_m). The temperature at which a double-stranded DNA molecule denatures into separate single strands. T_m is determined by the length of the molecule and its base composition. DNA s rich in G:C base pairs have higher T_m than A:T rich DNA, because since three hydrogen bonds are formed between G and C, but only two between A and T.

UN Food and Agriculture Organization — Glossary of Biotechnology for Food and Agriculture