pulsed-field gel electrophoresis 1 topic · 1 source Science› Biotechnology and GeneticsFood Biotechnology(Abbreviation: PFGE). A procedure used to separate very large (50 kbp to several Mbp) DNA molecules by alteRNA ting the direction of electric current in a pulsed manner across a gel.UN Food and Agriculture Organization — Glossary of Biotechnology for Food and Agriculture Cite