DNA ligase

1 topic · 2 sources

Food Biotechnology

Susan Allender-Hagedorn and Charles Hagedorn — An Agricultural and Environmental Biotechnology Annotated Dictionary

An enzyme that catalyses a reaction to link two separate DNA molecules via the formation of a phosphodiester bond between the 3'-hydroxyl end of one and the 5'-phosphate of the other. Its natural role lies in DNA repair and replication. An essential tool in recombinant DNA technology, as it enables the incorporation of foreign DNA into vectors.

UN Food and Agriculture Organization — Glossary of Biotechnology for Food and Agriculture