Sexual reproductionThe process where two cells (gametes) fuse to form one hybrid, fertilized cell. See Asexual reproduction, Gamete, Hybrid.
shake cultureAn agitated suspension in culture providing adequate aeration for cells in the liquid medium.
shakerA platform, with set or variable speed control, used to agitate vessels containing liquid cell cultures. Also described as a platform shaker.
ShearLiterally the sliding of one layer across another, with deformation and fracturing in the direction parallel to the movement.
Shine-Dalgarno sequenceA conserved sequence of prokaryotic mRNAs that is complementary to a sequence near the 5' terminus of the 16S ribosomal RNA and is involved in the…
shoot differentiationThe development of growing points, leaf primordia and finally shoots from a shoot tip, axial bud, or callus surface.
shoot tipThe terminal bud (0.1 - 1.0 mm) of a plant, which consists of the apical meristem (0.05 - 0.
shoot-tip graftA shoot tip or meristem tip grafted onto a prepared seedling or micropropagated rootstock in culture.
short interspersed nuclear element(Abbreviation: SINE ). Families of short (150-300 bp), moderately repetitive DNA elements of eukaryotic genomes.
short-day plantA plant which will not flower until triggered to do so by exposure to one or a number of dark periods equal to or longer than its critical period.
shotgun genome sequencingA strategy for sequencing a whole genome, in which the genomic DNA is initially fragmented into pieces small enough to be sequenced.
shuttle vectorA plasmid capable of replicating in two different host organisms because it carries two different origins of replication and can therefore be used to…
sib-matingThe deliberate crossing of siblings. Generally done where self-incompatibility prevents the production of self-fertilized progeny.
siderophoreA low molecular weight entity that binds very tightly to iron. Siderophores are synthesized by a variety of soil micro-organisms to ensure that the…
sieve cellA long and slender sieve element in vascular plants, characterized by relatively unspecialized sieve areas and tapering end walls that lack sieve plates.
sieve elementThe phloem cell concerned with longitudinal conduction of food materials.
sieve platePerforated wall area in a sieve tube element, through which strands connecting sieve tube protoplasts can pass.
sieve tubeA tube within the phloem tissue of a plant, composed of joined sieve elements.
sigma factorThe sub-unit of prokaryotic RNA polymerases responsible for the initiation of transcription at specific initiation sequences.
signal sequenceA stretch of 15-30 amino acid residues at the N terminus of a protein, which is thought to enable the protein to be secreted (pass through a cell…
signal transduction(SIG-nul trans-DUK-shun) The process by which a cell responds to substances in its environment.
Signal-to-Noise RatioA specifically produced response (signal) compared to the response level (noise) when no specific stimulus (activity) is present.
silencingLoss of gene expression either through an alteration in the of a structural gene, or its regulatory region; or because of interactions between its…
single node cultureCulture of separate lateral bud s, each carrying a piece of stem tissue.
single nucleotide polymorphism(... NOO-klee-oh-TIDE PAH-lee-MOR-fih-zum) The most common type of change in DNA (molecules inside cells that carry genetic information).
single primer amplification reaction(Abbreviation: SPAR). A PCR-based genotyping technique in which genomic template is amplified with a single primer.
single-strand DNA binding proteinA protein that coats single-stranded DNA, preventing renaturation and so maintaining the DNA in an extended state.
single-stranded DNA(Abbreviation: ssDNA). DNA molecules separated from their complementary strand, either by its absence or following denaturation.
single-stranded nucleic acidNucleic acid molecules consisting of only one polynucleotide chain. The genomes of many viruses are single-stranded DNA molecules, as are most…
Site-SpecificA term used to describe any process or enzyme which acts at a defined sequence within a DNA or RNA molecule.
site-specific mutagenesisThe induction of mutation s, by molecular biology techniques, in one or more specific nucleotide s within a defined coding sequence in order to create…
six-base cutterType II restriction endonucleases whose recognition site and cleavage site consist of a characteristic sequence of six nucleotide pairs.
small nuclear ribonucleoprotein(Abbreviation: snRNP). A complex of small nuclear RNA and nuclear protein, heavily involved in the post-transcriptional processing of mRNA, especially the…
small nuclear RNA(Abbreviation: snRNA ). RNA transcripts of 100-300 bp that associate with proteins to form small nuclear ribonucleoprotein particles.
Small nuclear RNA (snRNA)Short RNA transcripts of 100-300 bp that associate with proteins to form small nuclear ribonucleoprotein particles (snRNPs), which participate in RNA…
SNPThe most common type of change in DNA (molecules inside cells that carry genetic information).
soil ameliorationThe improvement of poor soils. Includes the fungal and bacterial break down of plant organic matter, to form humus; the release of minerals - such as…
Somatic cell(soh-MA-tik ...) Any of the body cells except the reproductive (germ) cells.
somatic cell embryogenesisThe process of differentiation of somatic embryos either from explant cells (direct embryogenesis), or from callus generated from explants (indirect…
Somatic cell gene therapyThe repair or replacement of a defective gene within somatic tissue. (See Somatic cell.)
somatic cell hybrid panelA panel of cells created by cell fusion, typically involving a reference species (e.g. hamster) and the species of interest (e.g.
somatic cell variantA somatic cell with unique characters not present in the other cells, and which could be selected for by an appropriate screen.
Somatic cellsCells of body tissues other than the germline.
somatic embryoAn organized embryo -like structure. Although morphologically similar to a zygotic embryo it is initiated from somatic plant cells.
somatic hybridizationNaturally occurring or induced fusion of somatic protoplasts or cells of two genetically different parents. The difference may be as wide as interspecific.
somatic hypermutationThe high frequency of mutation that occurs in the gene segments encoding the variable regions of immunoglobulins during the differentiation of B…
somatic reductionHalving of the chromosomal number of somatic cells; a possible method of producing "haploids" from somatic cells and calli by artificial means.
somatotropin(SOH-muh-toh-TROH-pin) A protein made by the pituitary gland that helps control body growth and the use of glucose and fat in the body.
sonicationDisruption of cells or DNA molecules by high frequency sound waves.
SOS responseThe synthesis of a whole set of DNA repair, recombination and replication proteins in bacteria suffering severe DNA damage (e.g.
source DNAThe DNA from an organism that contains a target gene, and used as the starting material in a cloning experiment.
source organismA bacterium, plant or animal from which DNA is purified and used in a cloning experiment.
Southern blotA nitrocellulose or nylon membrane to which DNA fragments previously separated by gel electrophoresis, have been transferred by capillary action.
Southern hybridizationA procedure in which a cloned, labelled segment of DNA is hybridized to DNA restriction fragments on a Southern blot.
Southern hybridization (Southern blotting)A procedure in which DNA restriction fragments are transferred from an agarose gel to a nitrocellulose filter, where the denatured DNA is then hybridized…
spacer sequenceA DNA sequence separating neighbouring genes; spacer sequences are not usually transcribed.
SPARAbbreviation for single primer amplification reaction.
spargerA device that introduces, into a bioreactor, air in the form of fine bubbles.
spatial autocorrelation statisticsA set of statistical parameters aimed to depict the spatial (geographical) pattern of genetic diversity in a population.
speciationThe evolutionary differentiation of a pre-existing species into one or more distinct species.
SpeciesOrganisms in the same genus that have similar characteristics.
specific combining ability(Abbreviation: SCA). A component of genetic variance calculable where a number of genotypes are intercrossed in all possible combinations.
specificityWhen referring to a medical test, specificity refers to the percentage of people who test negative for a specific disease among a group of people who do…
spent mediumAfter sub-culture, medium which is discarded because it has been depleted of nutrients, dehydrated, or accumulated toxic metabolic products.
sperm competitionCompetition between different spermatozoa to fertilize the of a single female.
sperm sexingThe separation of mammalian sperm into those bearing an X chromosome and those bearing a Y chromosome, in order to be able to produce, via artificial…
spermatidImmature spermatozoon. One of the four cells formed at the end of the second meiotic division in spermatogenesis.
spermatocyteThe premeiotic parental cell of the spermatids; the primary spermatocyte before the initiation of the first meiotic division; the secondary spermatocyte…
spermatogenesisThe series of cell divisions in the testis as a result of which the formation and the maturation of the male gametes (i.e. sperm) are achieved.
spermatogonium(pl.: spermatogonia) Primordial male germ cell. These can either divide by mitosis to produce daughter cells, or enter a growth phase and differentiate…
spermatozoon(Abbreviation: sperm). (pl.: spermatozoa) The mature, mobile gametic cell of male animals, produced in the testis.
spharoblastNodule of wood which can give rise to adventitious shoots with juvenile characteristics.