Molecular cloningThe biological amplification of a specific DNA sequence through mitotic division of a host cell into which it has been transformed or transfected.
Molecular geneticsThe study of the flow and regulation of genetic information between DNA, RNA, and protein molecules.
molecular marker(muh-LEH-kyoo-ler ...) A biological molecule found in blood, other body fluids, or tissues that is a sign of a normal or abnormal process, or of a…
MoleculeThe smallest particle of a substance that has all of the physical and chemical properties of that substance. Molecules are made up of one or more atoms.
mono-unsaturatesOils containing mono-unsaturated fatty acids (i.e. where one -CH2-CH2- group in the hydrocarbon chain is replaced by -CH=CH-).
Monoclonal antibodiesImmunoglobulin molecules of single- epitope specificity that are secreted by a clone of B cells.
monoclonal antibody(MAH-noh-KLOH-nul AN-tih-BAH-dee) A type of protein made in the laboratory that can locate and bind to substances in the body, including tumor cells.
monocotyledon(Abbreviation: monocot). A flowering plant whose embryo has one cotyledon. Examples are cereals (corn, wheat, rice etc.), banana, and lily.
MonocultureThe agricultural practice of cultivating crops consisting of genetically similar organisms.
monoeciousA plant species that has separate male and female flowers on the same plant (e.g. maize).
monosomicA form of aneuploidy in which a diploid organism lacks one member of a homologous chromosome pair.
monozygotic twinOne of a pair of twins derived from a single fertilized egg. Synonym: identical twin.
morphogenA substance that stimulates the development of form or structure in an organism.
morphogenesisThe development, through growth and differentiation, of form and structure in an organism.
morphogenic responseThe effect on the developmental history of a plant or its parts exposed to a given set of growth conditions or to a change in the environment.
Morphology(mor-FAH-loh-jee) The science of the form and structure of organisms (plants, animals, and other forms of life).
MosaicA web browser popular before the introduction of Netscape and Internet Explorer.
multi-copyDescribing plasmids which replicate to produce many copies per host bacterial cell.
Multi-locus probeA probe that hybridizes to a number of different sites in the genome of an organism. (See Probe.)
multigene familyA set of genes (not necessarily mapping to the same genomic location) that are related in nucleotide sequence and/or that produce polypeptides with…
multigenicTrait controlled by several genes, as opposed to monogenic. Synonym: polygenic.
multimerA protein made up of more than one polypeptide chain.
multiple allelesThe existence of more than two alleles at a locus in a population.
multiple arbitrary amplicon profilingA collective term for a number of related polymerase chain reaction techniques, all of which use arbitrary primers, and which generate a number of…
multiple ovulation and embryo transfer(Abbreviation: MOET). A technology by which a single female that usually produces only one or two offspring can produce a litter of offspring.
multiplex1. The simultaneous amplification of a number of amplicons in a single polymerase chain reaction, achieved by including more than one set of primers in…
multivalent vaccineA vaccine designed to elicit an immune response either to more than one infectious agent or to several different antigenic determinants of a single agent.
mutable geneA gene which has an unusually high rate of mutation.
Mutagen(MYOO-tuh-jen) Anything that causes a mutation (a change in the DNA of a cell).
mutagenesisInduction of heritable change(s) in the genetic constitution of a cell through alterations to its DNA.
mutantAn organism or an allele bearing a mutation. Usually applied when a characteristic change in phenotype can be recognized.
Mutation(myoo-TAY-shun) Any change in the DNA of a cell. Mutations may be caused by mistakes during cell division, or they may be caused by exposure to…
Mutation breedingCommonly used practices in plant breeding and other areas in which chemicals or radiation are applied to whole organisms, for example plants, or cells so…
mutation pressureA constant mutation rate that adds mutant genes to a population; repeated occurrences of mutations in a population.
narrow-host-range plasmidA plasmid that can replicate in one, or at most a few, different bacterial species.
narrow-sense heritabilityThe proportion of the phenotypic variance that is due to variation in breeding values; the proportion of the phenotypic variance that is due to additive…
National Science Foundation (NSF)A nonregulatory agency which has oversight of biotechnology research activities that the agency funds.
native proteinThe naturally occurring form of a protein.
Natural selectionThe differential survival and reproduc- tion of organisms with genetic characteristics that enable them to better utilize environmental resources.
necrosis(ne-KRO-sis) Refers to the death of living tissues.
negative autogenous regulationInhibition of the expression of a gene or set of co-ordinately regulated genes by the product of the gene or the product of one of the genes.
negative control systemA mechanism by which a regulatory protein is required to turn off gene expression.
negative selectionSelection against individuals possessing a certain character. Opposite: positive selection.
neomycin phosphotransferase II(Abbreviation: npt-II). An enzyme which detoxifies the antibiotic neomycin, used as a marker gene to select for successfully transformed cells in plant…
neoplasm(NEE-oh-PLA-zum) An abnormal mass of tissue that results when cells divide more than they should or do not die when they should.
nickTo break (or a break in) a phosphodiester bond in one of the strands of a double-stranded DNA molecule.
Nick translationA procedure for making a DNA probe in which a DNA fragment is treated with DNase to produce single-stranded nicks, followed by incorporation of…
nicked circleDuring the extraction of plasmid DNA from a bacterial cell, one strand of the DNA often becomes nicked.
Nicked circle (relaxed circle)During extraction of plasmid DNA from the bacterial cell, one strand of the DNA becomes nicked.
nif gene clusterGroup of bacterial genes responsible for the biological fixation of atmospheric nitrogen.
NIHA federal agency in the U.S. that conducts biomedical research in its own laboratories; supports the research of non-Federal scientists in universities…
nitrateThe form of nitrogen that can be used directly by plants; a major component of inorganic fertilizers.
nitrificationThe natural process in which nitrogen in plant and animal wastes is oxidized, first to nitrites and then to nitrates, through the action of soil-borne…
NitrocelluloseA membrane used to immobilize DNA, RNA, or protein, which can then be probed with a labeled sequence or antibody.
nitrogen assimilationThe incorporation of nitrogen into the cells of living organisms.
Nitrogen fixationThe conversion of atmospheric nitrogen to biologically usable nitrates.
Nitrogenous baseThe purines (adenine and guanine) and pyrimidines (cytosine, and thymine or uracil ) that are present in DNA and RNA.
Nitrogenous basesThe purines (adenine and guanine) and pyrimidines (thymine, cytosine, and uracil) that comprise DNA and RNA molecules.